Journal: Asian Journal of Pharmaceutical Sciences
Article Title: Macrophage membrane-mediated targeted drug delivery for treatment of spinal cord injury regardless of the macrophage polarization states
doi: 10.1016/j.ajps.2021.03.005
Figure Lengend Snippet: The influence of different macrophage polarization states. The expression of Mac-1 (A) and integrin α4 (B) in different macrophage subtypes were analyzed by flow cytometry; (C) The number of Mac-1 and integrin α4 molecules on a cell surface of macrophages by using a QuantumTMR-PE MESF kit to standardizing fluorescence intensity ( n = 3); (D) Representative fluorescence images of different liposomes (red) and HUVECs (blue) in the LPS-treated HUVECs (scale bar: 50 µm); (E) Quantification of the fluorescence intensity of preparations by using flow cytometry ( n = 3); (F) Representative ex vivo images of mice in 1, 4, 8 and 12 h after injection of DiR-labeled liposomes: RM-LIP/DiR (i), RM1-LIP/DiR (ii), RM2-LIP/DiR (iii).
Article Snippet: Quantum TM R-PE MESF Kit was obtained from Bangs Laboratories, Inc.(USA).
Techniques: Expressing, Flow Cytometry, Fluorescence, Liposomes, Ex Vivo, Injection, Labeling